1LTB

2.6 ANGSTROMS CRYSTAL STRUCTURE OF PARTIALLY-ACTIVATED E. COLI HEAT-LABILE ENTEROTOXIN (LT)

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	119.200, 98.200, 64.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.600
R-factor	0.172
Rwork	0.172
RMSD bond length	0.015
RMSD bond angle	3.240
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.086 *
Total number of observations	47662 *
Number of reflections	21707 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *		two-layer method, taken from Pronk, S.E. et al (1985). J. Biol. Chem., 260, 13580-13584. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	50 (mM)
2	1	1	EDTA	1 (mM)
3	1	1		3 (mM)
4	1	1	KF	0.3 (M)
5	1	1	precipitant	10 (mg/ml)
6	1	2	Tris-HCl	50 (mM)
7	1	2	EDTA	1 (mM)
8	1	2		3 (mM)
9	1	2	PEG6000	15 (%)