1LT9

Crystal Structure of Recombinant Human Fibrinogen Fragment D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	88.727, 94.511, 227.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.980 - 2.800
Rwork	0.224
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.110 *	0.540 *
Total number of observations	217604 *
Number of reflections	47742
<I/σ(I)>	14	3.1
Completeness [%]	99.9	99.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	4 *	9% PEG 3350, 70mM Calcium Chloride, 2mM Sodium Azide, 50mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
3	1	reservoir	Tris	50 (mM)	pH8.5
4	1	reservoir		2 (mM)
5	1	reservoir		70 (mM)
6	1	reservoir	PEG3350	9 (%(w/v))