1LT6
HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH METANITROPHENYLGALACTOSIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-09 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.670, 95.360, 85.650 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lta |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.500 * |
| Data reduction software | MACRO |
| Data scaling software | MACRO |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.298 |
| Number of reflections | 51781 | |
| Completeness [%] | 90.2 | 72 * |
| Redundancy | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | three-layered solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | ||
| 10 | 1 | 3 | 50 (mM) | ||
| 2 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 3 | 1 | 1 | 200 (mM) | ||
| 4 | 1 | 1 | EDTA | 0.5 (mM) | |
| 5 | 1 | 1 | azide | 0.1 (mM) | |
| 6 | 1 | 2 | thiodigalactoside | 200 (mM) | |
| 7 | 1 | 2 | Tris-HCl | 100 (mM) | |
| 8 | 1 | 3 | PEG6000 | 42 (%) | |
| 9 | 1 | 3 | Tris-HCl | 100 (mM) |
