Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 127.770, 127.770, 58.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.226 |
| R-free | 0.26800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1drm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.480 |
| High resolution limit [Å] | 2.400 * | 2.400 |
| Rmerge | 0.032 | 0.221 |
| Total number of observations | 26220 * | |
| Number of reflections | 7182 * | |
| <I/σ(I)> | 24.3 | 7 |
| Completeness [%] | 96.9 | 100 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | Gong, W., (1998) Proc. Natl. Acad. Sci. U.S.A., 95, 15177. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 4.5 (M) | ||
| 2 | 1 | reservoir | MPD | 5 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) |
