Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 127.770, 127.770, 58.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.226 |
R-free | 0.26800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1drm |
RMSD bond length | 0.016 |
RMSD bond angle | 1.730 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.480 |
High resolution limit [Å] | 2.400 * | 2.400 |
Rmerge | 0.032 | 0.221 |
Total number of observations | 26220 * | |
Number of reflections | 7182 * | |
<I/σ(I)> | 24.3 | 7 |
Completeness [%] | 96.9 | 100 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 4 * | Gong, W., (1998) Proc. Natl. Acad. Sci. U.S.A., 95, 15177. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 4.5 (M) | ||
2 | 1 | reservoir | MPD | 5 (%) | |
3 | 1 | reservoir | HEPES | 0.1 (M) |