1LSZ

CRYSTAL STRUCTURE OF THE MUTANT D52S HEN EGG WHITE LYSOZYME WITH AN OLIGOSACCHARIDE PRODUCT

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	79.300, 79.300, 37.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.16
Rwork	0.160
RMSD bond length	0.014 *
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.200
Rmerge	0.069 *
Total number of observations	89904 *
Number of reflections	7521
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.2 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GlcNAc6	1.5 (mg/ml)
2	1	drop	D52S HEWL	15 (mg/ml)
3	1	reservoir		4 (%)
4	1	reservoir	NaOAc	0.04 (M)