1LSY
CRYSTAL STRUCTURE OF THE MUTANT D52S HEN EGG WHITE LYSOZYME WITH AN OLIGOSACCHARIDE PRODUCT
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.300, 79.300, 37.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.900 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.064 * |
| Total number of observations | 17206 * |
| Number of reflections | 9294 |
| Completeness [%] | 93.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 * | 16 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GlcNAc6 | 1.5 (mg/ml) | |
| 2 | 1 | drop | D52S HEWL | 15 (mg/ml) | |
| 3 | 1 | reservoir | 4 (%) | ||
| 4 | 1 | reservoir | NaOAc | 0.04 (M) |
