1LSV
Crystal structure of the CO-bound BjFixL heme domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 127.010, 127.010, 58.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.215 |
| R-free | 0.25100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1drm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.315 |
| Total number of observations | 45212 * | |
| Number of reflections | 7132 * | |
| <I/σ(I)> | 14.4 | 6.8 |
| Completeness [%] | 99.8 | 96.6 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | Gong, W., (1998) Proc. Natl. Acad. Sci. U.S.A., 95, 15177. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 4.5 (M) | ||
| 2 | 1 | reservoir | MPD | 5 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) |
