1LSQ
RIBONUCLEASE A WITH ASN 67 REPLACED BY A BETA-ASPARTYL RESIDUE
Experimental procedure
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-04-01 |
| Detector | BRUKER NONIUS CAD4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.000, 37.800, 46.660 |
| Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5rsa |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOLEN |
| Data scaling software | MOLEN |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.051 |
| Total number of observations | 23907 * |
| Number of reflections | 17221 |
| Completeness [%] | 99.4 |
| Redundancy | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.7 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetic acid | 0.1 (M) | |
| 2 | 1 | 1 | ammonia | ||
| 3 | 1 | 1 | protein | 20 (mg/ml) | lyophilised |
