1LSL
Crystal Structure of the Thrombospondin-1 Type 1 Repeats
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00803, 1.00808, 1.0596 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.630, 84.410, 37.170 |
Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.235 |
Rwork | 0.238 |
R-free | 0.28200 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 27.854 * |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.075 | 0.469 |
Total number of observations | 162978 * | |
Number of reflections | 15485 * | |
<I/σ(I)> | 14.6 | 3.8 |
Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.5 M sodium potassium tartrate and 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.5 M sodium potassium tartrate and 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium potassium tartrate | 0.5 (M) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |