1LSG
THREE-DIMENSIONAL STRUCTURE OF THE PLATELET INTEGRIN RECOGNITION SEGMENT OF THE FIBRINOGEN GAMMA CHAIN OBTAINED BY CARRIER PROTEIN-DRIVEN CRYSTALLIZATION
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.900, 74.000, 30.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.400 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.32800 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.400 * |
Rmerge | 0.140 * |
Total number of observations | 15024 * |
Number of reflections | 4625 |
Completeness [%] | 90.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 2.5 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |