1LS9

Structure of the Cytochrome c6 from the Green Alga Cladophora glomerata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-05-17
Detector	MARRESEARCH
Wavelength(s)	0.8015
Spacegroup name	P 41 21 2
Unit cell lengths	46.063, 46.063, 100.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.684 - 1.300
R-factor	0.14922
Rwork	0.143
R-free	0.19000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctj
RMSD bond length	0.024
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14891.000 *	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.053 *
Total number of observations	114176 *
Number of reflections	27067
<I/σ(I)>	25.3	3.6
Completeness [%]	98.4	97.6
Redundancy	20.25	38.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	4 *	0.2 M Na-acetate, 0.1 M Na-cacodylate, pH 6.5, 30% w/v PEG 8000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.6
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5
5	1	reservoir	PEG8000	30 (%(w/v))