1LS9
Structure of the Cytochrome c6 from the Green Alga Cladophora glomerata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8015 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.063, 46.063, 100.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.684 - 1.300 |
| R-factor | 0.14922 |
| Rwork | 0.143 |
| R-free | 0.19000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctj |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14891.000 * | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.053 * | |
| Total number of observations | 114176 * | |
| Number of reflections | 27067 | |
| <I/σ(I)> | 25.3 | 3.6 |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 20.25 | 38.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 4 * | 0.2 M Na-acetate, 0.1 M Na-cacodylate, pH 6.5, 30% w/v PEG 8000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.6 |
| 3 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 5 | 1 | reservoir | PEG8000 | 30 (%(w/v)) |
