1LS2
Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI/PHILIPS EM420 |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 100 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 16.8 |
| Software | O,SPIDER |
| Symmetry | C1 |
