1LRO
Aquifex aeolicus KDO8P synthase H185G mutant in complex with PEP and Cadmium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-12-03 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.450, 84.450, 160.581 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
Rwork | 0.207 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 22.400 * |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.124 * | |
Total number of observations | 536046 * | |
Number of reflections | 60254 | |
<I/σ(I)> | 10.9 | |
Completeness [%] | 97.0 | 92.2 |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 4 * | Duewel, H.S., (2001) J.Biol.Chem., 276, 8393. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 30 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 5-6 (%) |