1LRM
Crystal structure of binary complex of the catalytic domain of human phenylalanine hydroxylase with dihydrobiopterin (BH2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.588, 108.117, 124.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| Rwork | 0.211 |
| R-free | 0.23500 |
| Structure solution method | Starting with 1PAH phases |
| Starting model (for MR) | 1pah |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.318 |
| Number of reflections | 26185 | |
| <I/σ(I)> | 11.1 | 23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 20% PEG 2000, 11% ethylene glycol 0.12M Na-Hepes pH 6.8, 5mM BH2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
