1LR7

Crystal structure of Fs1, the heparin-binding domain of follistatin, complexed with the heparin analogue sucrose octasulphate (SOS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	21.593, 38.153, 78.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.200 * - 1.500
R-factor	0.1905
Rwork	0.189
R-free	0.22200 *
Structure solution method	MAD
RMSD bond length	0.021 *
RMSD bond angle	1.940 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.070 *	0.200 *
Number of reflections	10456
Completeness [%]	95.0	71.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	18 *	30-35% 2-propanol, 0.6-0.7 M ammonium acetate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	2-propanol	30-35 (%)
3	1	reservoir	ammonium acetate	0.6-0.7 (M)
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.5