1LR6
Crystal structure of V45Y mutant of cytochrome b5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.404, 40.441, 39.196 |
Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
Resolution | 22.820 - 1.900 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.23500 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ehb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.165 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.820 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.062 | 0.194 |
Number of reflections | 7563 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 93.5 | 76.3 |
Redundancy | 3.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | phosphate | 3.1 (M) | pH7.5 |