1LQY
Crystal Structure of Bacillus stearothermophilus Peptide Deformylase Complexed with Antibiotic Actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-10 |
| Detector | MAC Science DIP-2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.013, 43.075, 92.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.900 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.705 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 * | 0.083 * |
| Number of reflections | 13516 | |
| <I/σ(I)> | 24.7 | 12.8 |
| Completeness [%] | 98.0 | 95.3 |
| Redundancy | 2.9 * | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 19 * | 7% PEG6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES-HCl | 25 (mM) | pH7.5 |
| 2 | 1 | drop | PEG6000 | 3.5 (%) | |
| 3 | 1 | drop | protein | 0.7 (mM) | |
| 4 | 1 | drop | actinonin | 0.7 (mM) | |
| 5 | 1 | reservoir | HEPPS-HCl | 50 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG6000 | 7 (%) |
