1LQX

Crystal structure of V45E mutant of cytochrome b5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-11-12
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	70.913, 40.338, 39.153
Unit cell angles	90.00, 111.57, 90.00

Refinement procedure

Resolution	22.910 - 1.800
R-factor	0.189
Rwork	0.189
R-free	0.22200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ehb
RMSD bond length	0.010
RMSD bond angle	22.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.910	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.219 *
Number of reflections	9048
<I/σ(I)>		5.7
Completeness [%]	93.6	74.5
Redundancy	3.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293	PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	3.6 (M)	pH7.5