1LQL
Crystal structure of OsmC like protein from Mycoplasma pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.360, 95.600, 308.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.850 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.091 * | 0.391 * |
| Number of reflections | 90144 | 6014 * |
| <I/σ(I)> | 23.7 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.0 * | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, sodium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.9 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 300 (mM) | ||
| 6 | 1 | reservoir | sodium iodide | 0.2 (M) | pH6.9 |
| 7 | 1 | reservoir | PEG3350 | 20 (%) |
