1LQJ
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 125.210, 125.210, 90.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.350 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.32600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uug |
| RMSD bond length | 0.014 |
| RMSD bond angle | 27.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.154 * | 0.369 * |
| Total number of observations | 38941 * | |
| Number of reflections | 20394 | |
| <I/σ(I)> | 6.9 | 1.4 |
| Completeness [%] | 90.2 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 20mM Tris-HCl, 100mM NaCl, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 293K, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 15 (%(w/v)) |
