1LQA
TAS PROTEIN FROM ESCHERICHIA COLI IN COMPLEX WITH NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.300, 81.460, 145.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 * |
| R-factor | 0.14634 |
| Rwork | 0.145 |
| R-free | 0.18600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.063 * | 0.175 |
| Number of reflections | 160862 * | |
| <I/σ(I)> | 23.8 | 4.1 |
| Completeness [%] | 92.0 * | 57.8 |
| Redundancy | 3.3 * | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris, 15% PEG8000, 50mM Ammonium Sulfate, 0.2M Magnesium Chloride, 10mM NADPH, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 2 | 1 | reservoir | PEG8000 | 15 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 50 (mM) | |
| 4 | 1 | reservoir | 0.2 (M) |
