1LQ1
DNA Complexed Structure of the Key Transcription Factor Initiating Development in Sporulation Bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.917, 83.301, 91.241 |
| Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.25900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 * | 0.385 * |
| Total number of observations | 243795 * | |
| Number of reflections | 32507 * | |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 1.0 | 78.2 * |
| Redundancy | 7.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 22 * | PEG3350, sodium chloride, calcium chloride, tris-HCl, iso-propanol, pH 7.0, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CaCl | ||
| 4 | 1 | 1 | tris-HCl | ||
| 5 | 1 | 1 | iso-propanol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 12 (%) | ||
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | reservoir | isopropanol | 2 (%) |
