1LPY

Multiple Methionine Substitutions in T4 Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 32 2 1
Unit cell lengths	60.220, 60.220, 93.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.700 *
R-factor	0.207 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Native T4 Lysozyme
RMSD bond length	0.017 *
RMSD bond angle	2.600
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.740
High resolution limit [Å]	1.700 *	1.650
Rmerge	0.043	0.103
Number of reflections	23682
<I/σ(I)>	7.1	6.4
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol