1LPY
Multiple Methionine Substitutions in T4 Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.220, 60.220, 93.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.700 * |
R-factor | 0.207 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Native T4 Lysozyme |
RMSD bond length | 0.017 * |
RMSD bond angle | 2.600 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.740 |
High resolution limit [Å] | 1.700 * | 1.650 |
Rmerge | 0.043 | 0.103 |
Number of reflections | 23682 | |
<I/σ(I)> | 7.1 | 6.4 |
Completeness [%] | 96.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | 0.25 (M) | ||
3 | 1 | reservoir | phosphate | 2.0 (M) | |
4 | 1 | reservoir | beta-mercaptoethanol |