1LPK
CRYSTAL STRUCTURE OF FXA IN COMPLEX WITH 125.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-04 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54128 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.702, 72.196, 78.612 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
Rwork | 0.192 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.490 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.065 * | 0.235 |
Total number of observations | 37057 * | |
Number of reflections | 15227 | |
<I/σ(I)> | 11.8 | 3.1 |
Completeness [%] | 89.8 | 63 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 19 * | PEG600, MES, CaCl2, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium sulfate | 0.6 (mM) | |
2 | 1 | drop | 1 (mg/ml) | ||
3 | 1 | drop | benzamidine | 10 (mg/ml) | |
4 | 1 | drop | protein | 60 (mg/ml) | |
5 | 1 | reservoir | ammonium sulfate | 1.2-1.6 (M) | |
6 | 1 | reservoir | pH5.0-7.0 |