1LPJ
Human cRBP IV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 34.053, 57.186, 67.939 |
Unit cell angles | 90.00, 104.03, 90.00 |
Refinement procedure
Resolution | 24.440 - 2.000 |
Rwork | 0.230 |
R-free | 0.27600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1crb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 * | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.124 | 0.382 |
Total number of observations | 19128 * | |
Number of reflections | 7611 | |
<I/σ(I)> | 3.8 | 1.3 |
Completeness [%] | 89.6 | 91.4 * |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 18 * | 10% PEG 4000, 10% isopropanol, 100 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
2 | 1 | reservoir | 2-propanol | 10 (%(v/v)) | |
3 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.6 |
4 | 1 | drop | protein | 8 (mg/ml) |