1LPF

THREE-DIMENSIONAL STRUCTURE OF LIPOAMIDE DEHYDROGENASE FROM PSEUDOMONAS FLUORESCENS AT 2.8 ANGSTROMS RESOLUTION. ANALYSIS OF REDOX AND THERMOSTABILITY PROPERTIES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	113.600, 66.400, 164.300
Unit cell angles	90.00, 99.20, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.192
Rwork	0.192
RMSD bond length	0.019
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.043 *
Total number of observations	40000 *
Number of reflections	23546 *
Completeness [%]	78.2 *	40.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	9 (%(w/v))
3	1	drop	HEPES	50 (mM)
4	1	drop	EDTA	0.5 (mM)
5	1	drop		0.02 (%(w/v))
6	1	reservoir	PEG6000	9 (%)