1LP8

HIGH RESOLUTION STRUCTURE OF RECOMBINANT DIANTHIN ANTIVIRAL PROTEIN-POTENT ANTI-HIV AGENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	2000-12-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	37.071, 78.559, 42.674
Unit cell angles	90.00, 105.30, 90.00

Refinement procedure

Resolution	8.000 - 1.650
R-factor	0.186
Rwork	0.180
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMOLOGY MODELING
RMSD bond length	0.005
RMSD bond angle	1.116
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.049	0.107
Number of reflections	26801
<I/σ(I)>	46.9
Completeness [%]	94.3	70.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	26% PEG3350, 0.1M CaCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K