1LP6
Crystal structure of orotidine monophosphate decarboxylase complexed with CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.130, 55.663, 65.838 |
Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.900 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 22.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.040 * | 0.100 * |
Total number of observations | 765425 * | |
Number of reflections | 32661 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | trisodium citrate, 1,2,3-heptanetriol, 12-crown-4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1,2,3-heptanetriol | 1.5-2 (%(w/v)) | |
2 | 1 | reservoir | 12-crown-4 | 5 (%(v/v)) | |
3 | 1 | reservoir | (+-)-1,3-butanediol | 10 (%) |