1LP3
The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 |
Unit cell lengths | 249.690, 249.690, 644.760 |
Unit cell angles | 90.00, 101.16, 120.00 |
Refinement procedure
Resolution | 39.800 - 3.000 |
R-factor | 0.338 |
Rwork | 0.338 |
R-free | 0.34200 |
Structure solution method | Molecular replacement at 15A, then phase extension by symmetry averaging |
Starting model (for MR) | PDB entries 4dpv 1dnv |
RMSD bond length | 0.020 |
RMSD bond angle | 26.680 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 * | 3.560 |
High resolution limit [Å] | 3.000 | 3.000 * |
Rmerge | 0.156 | 0.309 |
Total number of observations | 747988 * | |
Number of reflections | 495356 * | |
<I/σ(I)> | 6.3 | 5.9 |
Completeness [%] | 56.0 | 80 * |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.3 | 295 | PEG 6000, magnesium chloride, hepes, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.5 (mg/ml) | |
2 | 1 | drop | glycerol | 25 (%) | |
3 | 1 | reservoir | PEG6000 | 4-5 (%) |