1LOG

X-RAY STRUCTURE OF A (ALPHA-MAN(1-3)BETA-MAN(1-4)GLCNAC)-LECTIN COMPLEX AT 2.1 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	137.100, 63.300, 54.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.175
Rwork	0.175
RMSD bond length	0.015
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.059 *
Total number of observations	78639 *
Number of reflections	23795 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		taken from Bourne, Y. et al. (1990). J. Mol. Biol., 213, 211-213. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		2 (mM)
3	1	drop	disaccharide	136 (mM)
4	1	reservoir	MPD	36-44 (%)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir		0.1 (M)