1LNZ

Structure of the Obg GTP-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-24
Detector	CUSTOM-MADE
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	59.580, 105.006, 124.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.600
R-factor	0.203
Rwork	0.203
R-free	0.29300
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.360 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.600
Rmerge	0.079 *	0.320 *
Total number of observations	70639 *
Number of reflections	38652 *
Completeness [%]	82.5 *	46.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	7-12% isopropanol, 0.1M magnesium chloride, 0.05M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	isopropanol	7-12 (%)
6	1	reservoir		0.1 (M)
7	1	reservoir	sodium cacodylate	0.05 (M)	pH6.5