1LNY
Crystal structure of the recombinant mouse-muscle adenylosuccinate synthetase complexed with 6-phosphoryl-IMP, GDP and Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.500, 70.120, 105.130 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.200 |
| Rwork | 0.207 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j4b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 * |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.047 | 0.222 * |
| Total number of observations | 175127 * | |
| Number of reflections | 48646 * | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 92.3 * | 86.5 * |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | PEG 400, magnesium acetate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | drop | IMP | 5 (mM) | |
| 7 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 9 | 1 | reservoir | PEG3350 | 18-19 (%(w/v)) |
