1LNQ
CRYSTAL STRUCTURE OF MTHK AT 3.3 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 137.000, 137.000, 373.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.450 - 3.300 |
| R-factor | 0.299 * |
| Rwork | 0.301 |
| R-free | 0.31500 * |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 56550 | |
| <I/σ(I)> | 1 | |
| Completeness [%] | 95.0 | 71 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEGMME350, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEGMME350, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | PEGMME350, CaCl2, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | LDAO | 5 (mM) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | protein | 15.0 (mg/ml) | |
| 5 | 1 | reservoir | PEG350 MME | 23-26 (%) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 7 | 1 | reservoir | 200 (mM) |
