1LNF
A structural analysis of metal substitutions in thermolysin
Experimental procedure
| Experimental method | SINGLE CRYSTAL |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | MULTIWIRE AREA DETECTOR |
| Detector | XUONG-HAMLIN |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.850, 93.850, 131.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.152 |
| Structure solution method | MOLECULAR SUBSTITUTION |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.000 |
| Data reduction software | XENGEN ((HOWARD) |
| Data scaling software | XENGEN ((HOWARD) |
| Refinement software | TNT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 | Holmes, M.A., (1982) J.Mol.Biol., 160, 623. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 2.5 (M) | ||
| 2 | 1 | 2 | calcium acetate | 0.01 (M) | |
| 3 | 1 | 2 | Tris | 0.01 (M) | |
| 4 | 1 | 2 | dimethyl sulphoxide | 7 (%(v/v)) |
