1LN8

Crystal Structure of a New Isoform of Phospholipase A2 from Naja naja sagittifera at 1.6 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	200
Detector technology	CCD
Collection date	2001-09-14
Detector	MARRESEARCH
Wavelength(s)	0.80200
Spacegroup name	P 41
Unit cell lengths	42.099, 42.099, 64.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.18558
Rwork	0.184
R-free	0.22193
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LFF
RMSD bond length	0.008
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	12931
<I/σ(I)>	18.2	3
Completeness [%]	99.0	99.1
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	sodium phosphate, ethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K