1LN4
CRYSTAL STRUCTURE OF E. COLI YHBY
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9611,0.9793,0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.324, 57.324, 70.895 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.22300 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 2.800 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | TNT (5F) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.074 | 0.139 |
Number of reflections | 22061 * | |
<I/σ(I)> | 4.5 | 4.5 |
Completeness [%] | 100.0 * | 99.9 |
Redundancy | 10.6 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | PEG 200, sodium chloride, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | PEG 200, sodium chloride, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 50 (mM) | ||
2 | 1 | drop | Tris | 10 (mM) | pH7.0 |
3 | 1 | drop | EDTA | 0.1 (mM) | |
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | protein | 10 (mg/ml) | |
6 | 1 | reservoir | PEG200 | 45 (%(v/v)) | |
7 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
8 | 1 | reservoir | 100 (mM) |