1LN0
Structure of the Catalytic Domain of Homing Endonuclease I-TevI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-01 |
Detector | BRANDEIS - B1.2 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 123.870, 56.580, 35.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.25500 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.034 | 0.124 |
Number of reflections | 16242 * | |
<I/σ(I)> | 33.4 | 8.3 |
Completeness [%] | 92.2 | 61.6 |
Redundancy | 3.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | 22% PEG8000, 0.1 M MES, 5% ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | 0.01 (%(w/v)) | ||
6 | 1 | reservoir | PEG8000 | 22 (%(w/v)) | |
7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
8 | 1 | reservoir | ammonium sulfate | 5 (%(w/v)) |