1LMQ

THE CRYSTAL STRUCTURES OF THREE COMPLEXES BETWEEN CHITOOLIGOSACCHARIDES AND LYSOZYME FROM THE RAINBOW TROUT

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1994-07
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	76.580, 76.580, 54.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.165
RMSD bond length	0.012
RMSD bond angle	0.040
Data reduction software	MADNES
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	14.650 *
High resolution limit [Å]	1.600 *
Rmerge	0.033
Number of reflections	22072
Completeness [%]	89.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	saccaride	10 (mg/ml)
2	1	drop	enzyme	18 (mg/ml)
3	1	drop	ammonium sulfate	27.5-32.5 (%sat)
4	1	reservoir	acetate	0.1 (M)
5	1	reservoir	citrate	0.1 (M)
6	1	reservoir	phosphate	0.1 (M)