1LMP
THE CRYSTAL STRUCTURES OF THREE COMPLEXES BETWEEN CHITOOLIGOSACCHARIDES AND LYSOZYME FROM THE RAINBOW TROUT
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-08 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 76.420, 76.420, 54.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.159 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.047 |
| Data reduction software | MADNES |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 14.670 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.033 |
| Number of reflections | 12175 |
| Completeness [%] | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 277 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | saccaride | 10 (mg/ml) | |
| 2 | 1 | drop | enzyme | 18 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 27.5-32.5 (%sat) | |
| 4 | 1 | reservoir | acetate | 0.1 (M) | |
| 5 | 1 | reservoir | citrate | 0.1 (M) | |
| 6 | 1 | reservoir | phosphate | 0.1 (M) |
