1LMC

THE CRYSTAL STRUCTURE OF A COMPLEX BETWEEN BULGECIN, A BACTERIAL METABOLITE, AND LYSOZYME FROM THE RAINBOW TROUT

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1994-03-17
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	76.570, 76.570, 54.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.163
RMSD bond length	0.017
RMSD bond angle	0.049
Data reduction software	MADNES
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	14.680 *
High resolution limit [Å]	2.000 *
Rmerge	0.059
Number of reflections	10895
Completeness [%]	86.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		277 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	bulgecin	10 (mg/ml)
2	1	drop	ammonium sulfate	30-45 (%sat)
3	1	reservoir	acetate	0.1 (M)
4	1	reservoir	citrate	0.1 (M)
5	1	reservoir	phosphate	0.1 (M)