1LM7

Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	48.293, 99.773, 51.687
Unit cell angles	90.00, 97.58, 90.00

Refinement procedure

Resolution	100.000 * - 3.000
R-factor	0.249
Rwork	0.249
R-free	0.29600
Structure solution method	SAD
RMSD bond length	0.095 *
RMSD bond angle	1.460 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000 *	3.000
Rmerge	0.087 *	0.230 *
Total number of observations	208172 *
Number of reflections	9183 *
Completeness [%]	93.8	84.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	20 *	PEG1500, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	reservoir	PEG1500	35 (%(w/v))