1LLS
CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-31 |
Detector | ADSC |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 38.207, 44.026, 57.649 |
Unit cell angles | 100.75, 101.36, 102.62 |
Refinement procedure
Resolution | 17.070 - 1.800 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22600 |
Structure solution method | Isomorphous with Structure in Database |
Starting model (for MR) | 1omp |
RMSD bond length | 0.010 |
RMSD bond angle | 22.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.070 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.029 * | |
Total number of observations | 55908 * | |
Number of reflections | 29855 | |
<I/σ(I)> | 19.9 | 10.4 |
Completeness [%] | 92.3 | 76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 10 mM Sodium Citrate, 23% PEG 8000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 16 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 10 (mM) | |
3 | 1 | reservoir | PEG8000 | 23 (%(w/v)) | pH6.6 |