1LLO
HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.330, 57.990, 82.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.850 |
R-factor | 0.146 |
R-free | 0.20000 |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.820 * | 1.820 * |
Rmerge | 0.039 | 0.149 * |
Total number of observations | 91378 * | |
Number of reflections | 22218 * | |
Completeness [%] | 98.7 * | 0.732 * |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4 | 277 * | pH 4.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | BES | 20 (mM) | |
3 | 1 | reservoir | sodium chloride | 1.7-3.4 (M) |