1LL3

Crystal Structure of Rabbit Muscle Glycogenin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	108
Detector technology	CCD
Collection date	2001-06-04
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	59.129, 104.830, 120.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.212 *
Rwork	0.210
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	23.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.559
Total number of observations	162821 *
Number of reflections	29807 *
<I/σ(I)>	23.1	2.4
Completeness [%]	98.4 *	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	4 *	8-10 mg/mL glycogenin, 0.7 to 1.2 M ammonium sulphate, and 100 mM sodium phosphate buffer pH 6.6 to 6.9, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	glycogenin	8-10 (mg/ml)
2	1	reservoir	ammonium sulfate	0.7-1.2 (M)
3	1	reservoir	sodium phosphate	100 (mM)	pH6.6-6.9