1LKZ
Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coli.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97193 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.335, 71.809, 193.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.500 |
R-factor | 0.227 * |
Rwork | 0.227 |
R-free | 0.27000 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 25.000 * |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.580 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 * | |
Total number of observations | 89423 * | |
Number of reflections | 29225 | |
<I/σ(I)> | 16.9 | |
Completeness [%] | 87.9 | 73.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 295 | Ammonium sulfate, Isopropanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.7 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | pH8.5 |
3 | 1 | drop | 400 (mM) | ||
4 | 1 | drop | glycerol | 5 (%(v/v)) | |
5 | 1 | reservoir | trisodium citrate | 1.05 (M) | |
6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |