1LKV
Crystal Structure of the Middle and C-terminal Domains of the Flagellar Rotor Protein FliG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.99981 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 113.943, 113.943, 128.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.000 * - 2.800 |
R-factor | 0.2574 |
Rwork | 0.255 |
R-free | 0.28000 * |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.972 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.035 * | 2.900 |
High resolution limit [Å] | 2.800 * | 2.800 |
Rmerge | 0.084 * | 0.235 * |
Total number of observations | 179161 * | |
Number of reflections | 12745 * | |
Completeness [%] | 96.0 * | 90.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 298 | Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 298 | Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 298 | Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | reservoir | isopropanol | 8-10 (%(v/v)) | |
5 | 1 | reservoir | 200-400 (mM) | ||
6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.4-5.0 |
7 | 1 | reservoir | pluronic F-68 | 1 (%) |