1LKS

HEN EGG WHITE LYSOZYME NITRATE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-09-15
Detector	RIGAKU RAXIS
Spacegroup name	P 1
Unit cell lengths	31.970, 65.080, 27.250
Unit cell angles	94.57, 111.56, 82.98

Refinement procedure

Resolution	20.000 - 1.100
R-factor	0.0989 *
R-free	0.14160 *
Data reduction software	MSC (SOFTWARE)
Data scaling software	MSC
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.100
Rmerge	0.095
Total number of observations	216397 *
Number of reflections	36397
Completeness [%]	78.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.7 *		THE CRYSTALS WERE GROWN IN THE PRESENCE OF BROMO PHENYL BLUE (BPB), AND THE CRYSTALS WERE DARK BLUE, ALMOST BLACK. HOWEVER, NO RELIABLE TRACE OF THE BPB WAS FOUND.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	acetate	100 (mM)
2	1	2	protein	10 (mg/ml)
3	1	2	sodium iodide	5 (%(w/w))