1LKR
MONOCLINIC HEN EGG WHITE LYSOZYME IODIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.894, 63.153, 60.227 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.1404 |
| R-free | 0.14040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MONOCLINIC LYSOZYME NITRATE (SUNDARALINGAM) |
| Data reduction software | MCS |
| Data scaling software | MCS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.076 |
| Total number of observations | 68962 * |
| Number of reflections | 22327 |
| Completeness [%] | 63.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 1% PROTEIN, 200 MMOLAR ACETATE BUFFER, PH 8, 5% SODIUM IODIDE, pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetate | 100 (mM) | |
| 2 | 1 | 1 | sodium hydroxide | 1.0 (M) | |
| 3 | 1 | 1 | protein | 10 (mg/ml) | |
| 4 | 1 | 1 | sodium iodide | 5 (%(w/w)) |
