1LK7
Structure of D-Ribose-5-Phosphate Isomerase from in complex with phospho-erythronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9326 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.689, 114.923, 119.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.2045 |
| Rwork | 0.202 |
| R-free | 0.27000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lk5 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.320 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | 0.387 * |
| Number of reflections | 67418 | |
| Completeness [%] | 96.3 * | 96.2 * |
| Redundancy | 4.0 * | 3.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 0.1M acetate buffer, 2.0M NaCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 5 | 1 | reservoir | 2.0 (M) |
