1LJJ
CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 10% TREHALOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.986, 62.758, 60.207 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21300 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.200 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 * | 0.157 * |
| Total number of observations | 51393 * | |
| Number of reflections | 13471 * | 1293 * |
| Completeness [%] | 95.1 * | 93.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | 293 | Sodium Acetate (0.1M) - Buffer, Sodium Nitrate (0.44M) - Precipitant, Trehalose(0.29M) - Additive; pH 4.6, LIQUID DIFFUSION at 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium acetate | 0.1 (M) | pH4.6 |
| 2 | 1 | 1 | trehalose | 10 (%) |
